Environmental Chemistry: Legiolert

How the IDEXX Legiolert test works

The Legiolert test detects Legionella pneumophila in water samples via bacterial enzyme detection technology. Due to the rich supply of nutrients within the Legiolert reagent, Legionella pneumophila cells grow and reproduce rapidly. Actively growing strains of Legionella pneumophila use an added substrate (present in the Legiolert reagent) to produce a brown color that signals the presence of Legionella pneumophila.

The Legiolert test detects Legionella pneumophila at 1 organism in 100 mL within 7 days.

Customers wishing to collect samples for Legionella pneumophila testing using Legiolert should follow these instructions:

  • Contact Environmental Sample Receiving (801-965-2405) and arrange to pick up (or have delivered) the 100 mL sterile sample bottles
  • Do not rinse the bottle or touch the lip of the bottle.
  • Allow source to flow for a few minutes until water temperature stabilizes. Slowly fill to above the 100 mL line.
  • Fill out the information on the sample container label.
  • Refrigerate or store on ice. Do not allow sample to freeze.
  • Return sample to the lab within 24 hours of collection.
  • Fill out the test request form with the required information.
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